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KMID : 1025520090510020123
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Journal of Animal Science and Technology
2009 Volume.51 No. 2 p.123 ~ p.128
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The 3rd Generation Genome Map of the Korean Cattle (Hanwoo)
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Lee Yong-Seok
Choi In-Ho
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Abstract
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Recently, the 2nd generation genome map of the Korean cattle(Hanwoo) has been constructed by comparison of the nucleotide sequence of the Korean cattle BAC clones with whole genome sequence of the bovine data-base(B_tau 2.1 build). The objective of this study was to update the 2nd generation genome map of the Korean cattle using the similar approach. The nucleotide sequence of the Korean cattle BAC clones utilized in the construction of the 2nd generation map was compared with the newly released bovine data-base(B_tau 3.1 build) to generate the 3rd generation map. While, 5,105 BAC clones were localized on bovine chromosome in the 2nd generation map, a total of 9,595 BAC clones, which spans about 37.27% of the bovine chromosome after eliminating the overlapping sequence among the clones, have been mapped on the bovine chromosome in the 3rd generation map. Further analysis of the nucleotide sequence of the BAC clones will allow us to develop map and facilitate to pinpoint the genes that are important for the improvement of the performance in this cattle breed.
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KEYWORD
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Korean cattle (Hanwoo), BAC End Sequence, BES, chromosome map
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